1. Use the Low Input Library Prep Kit (Cat. No. 634947). This unique adapter addition method does not allow for erroneous ligation. 2. Use the Nextera® XT DNA Sample Preparation Kit from Illumina to prepare your library. We recommend using an input amount of 100–150 pg amplified DNA. 3. Sequence only from Read Primer 1.
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We are continuously improving our protocols. Updates will be regularly posted here with detailed information on the improvements. Below are our main protocols for performing CITE-seq and Cell Hashing, specifically on Drop-seq or 10x Genomics single cell 3P chemistry (v2 and v3), followed by supplementary information and supplementary protocols for other technologies or instruments.